Novel Selective Inhibitor of .ALPHA.-Amylase Isoenzymes.
نویسندگان
چکیده
منابع مشابه
Determinations of amylase isoenzymes in serum by use of a selective inhibitor.
A simple, rapid screening procedure for determining the relative amounts of pancreatic (P)- and salivary (S)-type amylase in serum is presented, involving incorporation of a selective inhibitor (from wheat-germ) into commercially available BMD Single-Vial Amylase and Beckman Enzymatic Amylase-DS procedures for manual and automated isoamylase measurements. Optimal concentrations of inhibitor inh...
متن کاملAutomated measurement of amylase isoenzymes with 4-nitrophenyl-maltoheptaoside as substrate and use of a selective amylase inhibitor.
We automated a kinetic procedure for determining amylase isoenzymes in serum and urine samples. We used 4-nitro-phenylmaltoheptaoside as substrate and a selective amylase inhibitor with the Abbott-VP bichromatic system. By use of the maximum differences between pancreatic (P) and salivary (S) amylase activities remaining after inhibition by the selective inhibitor and by use of the linear range...
متن کاملA novel alpha-amylase inhibitor from amaranth (Amaranthus hypocondriacus) seeds.
The major alpha-amylase inhibitor (AAI) present in the seeds of Amaranthus hypocondriacus, a variety of the Mexican crop plant amaranth, is a 32-residue-long polypeptide with three disulfide bridges. Purified AAI strongly inhibits the alpha-amylase activity of insect larvae (Tribolium castaneum and Prostephanus truncatus) and does not inhibit proteases and mammalian alpha-amylases. AAI was sequ...
متن کاملAntenatal development of amylase isoenzymes.
Relatively little is known about the normal physiology of amniotic fluid, its dynamics, and its constituent enzymes and isoenzymes. The antenatal diagnosis of some metabolic errors is frequently based upon the investigation for the presence or absence of an enzyme in the amniotic fluid or cells. In establishing an antenatal diagnosis of this kind, it is essential that 2 major factors be borne i...
متن کاملCharacterization of Alpha-amylase inhibitor in Vigna sublobata
Alpha-amylase inhibitor protein, which inhibits the activity of insect (Callosobruchus analis) α-amylase, was characterized from V. sublobata. The molecular weight of purified inhibitor protein was 14 kDa by SDS-PAGE. The inhibitor is non-glycosylated protein and its N-terminal sequence is similar (A P S P V...) to Phaseolus vulgaris α-AI-1. Its pI value is 6.0 and largely localised in cotyledo...
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ژورنال
عنوان ژورنال: Biological and Pharmaceutical Bulletin
سال: 1994
ISSN: 0918-6158,1347-5215
DOI: 10.1248/bpb.17.993